comparison of radiation-induced bystander effect in qu-db cells after acute and fractionated irradiation: an in vitro study
نویسندگان
چکیده
objective: radiation effects induced in non-irradiated cells are termed radiation-induced bystander effects (ribe). the present study intends to examine the ribe response of qu-db bystander cells to first, second and third radiation fractions and compare their cumulative outcome with an equal, single acute dose. materials and methods: this experimental study irradiated three groups of target cells for one, two and three times with 60co gamma rays. one hour after irradiation, we transferred their culture media to non-irradiated (bystander) cells. we used the cytokinesis block micronucleus assay to evaluate ribe response in the bystander cells. the numbers of micronuclei generated in bystander cells were determined. results: ribe response to single acute doses increased up to 4 gy, then decreased, and finally at the 8 gy dose disappeared. the second and third fractions induced ribe in bystander cells, except when ribe reached to the maximum level at the first fraction. we split the 4 gy acute dose into two fractions, which decreased the ribe response. however, fractionation of 6 gy (into two fractions of 3 gy or three fractions of 2 gy) had no effect on ribe response. when we split the 8 gy acute dose into two fractions we observed ribe, which had disappeared following the single 8 gy dose. conclusion: the impact of dose fractionation on ribe induced in qu-db cells depended on the ribe dose-response relationship. where ribe increased proportionally with the dose, fractionation reduced the ribe response. in contrast, at high doses where ribe decreased proportionally with the dose, fractionation either did not change ribe (at 6 gy) or increased it (at 8 gy).
منابع مشابه
Comparison of Radiation-Induced Bystander Effect in QU-DB Cells after Acute and Fractionated Irradiation: An In Vitro Study
OBJECTIVE Radiation effects induced in non-irradiated cells are termed radiation-induced bystander effects (RIBE). The present study intends to examine the RIBE response of QU-DB bystander cells to first, second and third radiation fractions and compare their cumulative outcome with an equal, single acute dose. MATERIALS AND METHODS This experimental study irradiated three groups of target ce...
متن کاملThe role of dose fractionation in the level of Radiation- Induced Bystander Effect in QU-DB Cells
Introduction: Radiation effects induced in non-irradiated cells are termed radiation- induced bystander effects (RIBE). The present study intends to examine the RIBE response of QU-DB bystander cells to first, second and third radiation fractions and compare their cumulative outcome with an equal, single acute dose. Materials and Methods: This experimental ...
متن کاملcost benefits of rehabilitation after acute coronary syndrome in iran; using an epidemiological model
چکیده ندارد.
Radiation Induced Bystander Effect
Introduction: Radiation effects observed in cells that are not irradiated are known as non-targeted effects. Radiation induced bystander effect (RIBE) as a kind of non-targeted effect has been introduced in recent years. RIBE occurs in unexposed cells which are related to adjacent or distant irradiated cells. RIBE contradict with "target theory" which necessitates radiation tr...
متن کاملAn Investigation of the Effects of Raw Garlic on Radiation-induced Bystander Effects in MCF7 Cells
Introduction Radiation-induced bystander effect (RIBE) is a phenomenon in which radiation signals are transmitted from irradiated cells to non-irradiated ones, inducing radiation effects in these cells. RIBE plays an effective role in radiation response at environmentally relevant low doses and in radiotherapy, given its impact on adjacent normal tissues or those far from the irradiated tumor. ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
cell journalجلد ۱۸، شماره ۳، صفحات ۳۴۶-۳۵۲
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023